human bladder transitional cell carcinoma cell lines  (ATCC)

Journal: Cancer Research

Article Title: Aberrant Nuclear Export of circNCOR1 Underlies SMAD7-Mediated Lymph Node Metastasis of Bladder Cancer

doi: 10.1158/0008-5472.CAN-21-4349

Figure Lengend Snippet: circNCOR1 inhibits LN metastasis of bladder cancer by inhibiting the TGFβ–SMAD signaling pathway. A – C, Representative images ( A ) and quantification of the tube formation ( B ) and Transwell migration ( C ) by HLECs cocultured with UM-UC-3 cells. Scale bars, 100 μm. D, The popliteal LN metastatic rate in the nude mice ( n = 12 per group). E and F, Bioluminescence ( E ) and LN volume ( F ) of popliteal LN metastasis in the nude mice ( n = 12 per group). G, Luciferase activity of the SMAD7 promoter in indicated UM-UC-3 cells. H, qRT-PCR analysis of SMAD7 expression in indicated UM-UC-3 cells. I and J, Western blotting of SMAD7 expression in indicated UM-UC-3 ( I ) and T24 ( J ) cells. The statistical difference was assessed by the two-tailed Student t test in B , C , E , and F , and the χ 2 test in D ; and one-way ANOVA followed by Dunnett tests in G and H . Error bars show the SD from three independent experiments. **, P < 0.01.

Article Snippet: The human urinary bladder transitional cell carcinoma cell lines T24 (RRID: CVCL_0554), UM-UC-3 (RRID: CVCL_1783) were purchased from ATCC, RT112 (RRID: CVCL_1670) was purchased from German Collection of Microorganisms and Cell Cultures GmbH (DSMZ) and UM-UC-1 (RRID: CVCL_2743) was purchased from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Migration, Luciferase, Activity Assay, Quantitative RT-PCR, Expressing, Western Blot, Two Tailed Test

Journal: Translational Andrology and Urology

Article Title: The effects of modified RNA-binding proteins HuR on the biological behavior of the bladder cancer T24 cell line

doi: 10.21037/tau-22-123

Figure Lengend Snippet: Human antigen R (HuR) promotes the proliferation of T24 cells. Compared to the control group, after 48 h, cell viability was significantly increased in the HuR overexpression group and significantly decreased in the HuR knockdown group (F=1929.061, P<0.01). *, P<0.05.

Article Snippet: Experimental materials The T24 human bladder transitional cell carcinoma cell line was purchased from Procell Co., Ltd., and mycoplasma testing was conducted for all cells.

Techniques: Control, Over Expression, Knockdown

Journal: Translational Andrology and Urology

Article Title: The effects of modified RNA-binding proteins HuR on the biological behavior of the bladder cancer T24 cell line

doi: 10.21037/tau-22-123

Figure Lengend Snippet: Human antigen R (HuR) promotes the migration of T24 cells. Compared to the control group, the number of migrated cells of HuR overexpression group was significantly increased and significantly decreased in the HuR knockdown group (F=145.982, P<0.01, all the photos magnified at ×400; all the cells were stained by crystal violet staining). *, P<0.05. (A) Control group; (B) HuR overexpression group; (C) HuR knockdown group; (D) the number of cell migrations of each group.

Article Snippet: Experimental materials The T24 human bladder transitional cell carcinoma cell line was purchased from Procell Co., Ltd., and mycoplasma testing was conducted for all cells.

Techniques: Migration, Control, Over Expression, Knockdown, Staining

Journal: Translational Andrology and Urology

Article Title: The effects of modified RNA-binding proteins HuR on the biological behavior of the bladder cancer T24 cell line

doi: 10.21037/tau-22-123

Figure Lengend Snippet: Human antigen R (HuR) inhibits T24 cell apoptosis. Compared to the control group, the apoptotic rate was significantly decreased in the HuR overexpression group and significantly increased in the knockdown group (F=665.452, P<0.01). *, P<0.05. (A) Control group; (B) HuR overexpression group; (C) HuR knockdown group; (D) each group apoptosis rate of T24 cell.

Article Snippet: Experimental materials The T24 human bladder transitional cell carcinoma cell line was purchased from Procell Co., Ltd., and mycoplasma testing was conducted for all cells.

Techniques: Control, Over Expression, Knockdown